Application
ERI1 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the ERI1 antibody detected the ERI1 protein (arrow).| Product Name | ERI1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ERI1; 3'EXO; THEX1; 3'-5' exoribonuclease 1; 3'-5' exonuclease ERI1; Eri-1 homolog; Histone mRNA 3'-end-specific exoribonuclease; Histone mRNA 3'-exonuclease 1; Protein 3'hExo |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | 3'EXO THEX1 |
|---|---|
| Alternative Names | ERI1 3'EXO THEX1 3'-5' exoribonuclease 1 3'-5' exonuclease ERI1 Eri-1 homolog Histone mRNA 3'-end-specific exoribonuclease Histone mRNA 3'-exonuclease 1 Protein 3'hExo |
| Molecular Weight(MW) | 40064 Da |
| Function | RNA exonuclease that binds to the 3'-end of histone mRNAs and degrades them, suggesting that it plays an essential role in histone mRNA decay after replication. A 2' and 3'-hydroxyl groups at the last nucleotide of the histone 3'-end is required for efficient degradation of RNA substrates. Also able to degrade the 3'-overhangs of short interfering RNAs (siRNAs) in vitro, suggesting a possible role as regulator of RNA interference (RNAi). Requires for binding the 5'-ACCCA-3' sequence present in stem-loop structure. Able to bind other mRNAs. Required for 5.8S rRNA 3'-end processing. Also binds to 5.8s ribosomal RNA. Binds with high affinity to the stem-loop structure of replication- dependent histone pre-mRNAs |
| Tissue Specificity | This ERI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 79-109 amino acids from the N-terminal region of human ERI1. |
| Cellular Localization | Cytoplasm. Nucleus. Nucleus, nucleolus. |
| Entrez Gene | 90459 |
|---|---|
| Nucleotide Accession | NP_699163.2 |
Application
ERI1 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the ERI1 antibody detected the ERI1 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
