Application
Figure 1: Western blot analysis using ERN1 mouse mAb against Raji (1), A431 (2), Jurkat(3), Hela(4) and HEK293(5)cell lysate.Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human brain tissue (A) and stomach tissue (B), showing cytoplasmic localization using ERN1 mouse mAb with DAB staining.| Product Name | ERN1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IRE1; IRE1P; IRE1a; hIRE1p; FLJ30999; MGC163277; MGC163279; ERN1 |
| Conjugation | Agarose |
| Modification | Acetylated |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 9F2 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500 - 2000. IHC |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | IRE1 |
|---|---|
| Alternative Names | IRE1 IRE1P IRE1a hIRE1p FLJ30999 MGC163277 MGC163279 ERN1 |
| Molecular Weight(MW) | 109735 Da |
| Function | Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto- activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis |
| Tissue Specificity | Ubiquitously expressed. High levels observed in pancreatic tissue |
| Cellular Localization | Endoplasmic reticulum membrane; Single-pass type I membrane protein |
| Entrez Gene | 2081 |
|---|---|
| Nucleotide Accession | NP_001424.3 |
Application
Figure 1: Western blot analysis using ERN1 mouse mAb against Raji (1), A431 (2), Jurkat(3), Hela(4) and HEK293(5)cell lysate.
Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human brain tissue (A) and stomach tissue (B), showing cytoplasmic localization using ERN1 mouse mAb with DAB staining.| Application Notes | WB:1:500 - 2000. IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of human ERN1(aa282-433) expressed in E. Coli. |
