Application
ERO1LB Antibody (C-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane). This demonstrates the ERO1LB antibody detected the ERO1LB protein (arrow).Application
ERO1LB Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded mouse testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ERO1LB Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
ERO1LB Antibody (C-term) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ERO1LB Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | ERO1LB ERO1-like protein beta Endoplasmic oxidoreductin-1-like protein B Oxidoreductin-1-L-beta |
|---|---|
| Molecular Weight(MW) | 53543 Da |
| Function | Essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. Acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. Does not act as a direct oxidant of folding substrate, but relies on P4HB/PDI to transfer oxidizing equivalent Associates with ERP44 but not with GRP54, demonstrating that it does not oxidize all PDI related proteins and can discriminate between PDI and related proteins. Its reoxidation probably involves electron transfer to molecular oxygen via FAD. Acts independently of glutathione. May be responsible for a significant proportion of reactive oxygen species (ROS) in the being a source of oxidative stress. Required for the folding of cell, thereby being a source of oxidative stress |
| Tissue Specificity | Widely expressed at low level. Expressed at high level in lower digestive tract. Highly expressed in stomach and duodenum. Weakly expressed in esophagus |
| Cellular Localization | Endoplasmic reticulum membrane; Peripheral membrane protein; Lumenal side. Note=The association with ERP44 may be essential for its retention in the endoplasmic reticulum |
| Entrez Gene | 56605 |
|---|
Application
ERO1LB Antibody (C-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane). This demonstrates the ERO1LB antibody detected the ERO1LB protein (arrow).
Application
ERO1LB Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded mouse testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ERO1LB Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
ERO1LB Antibody (C-term) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
