Application
Western blot analysis of ERP29 Antibody (Center) in A2058,A375,MCF7,NCI-H460,Y79 cell line lysates (35ug/lane). ERP29 (arrow) was detected using the purified Pab.Application
Western blot analysis of ERP29 (arrow) using rabbit polyclonal ERP29 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ERP29 gene (Lane 2) .Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ERP29 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
ERP29 Antibody (Center) flow cytometric analysis of NCI-H292 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ERP29 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | C12orf8, ERP28 |
|---|---|
| Alternative Names | ERP29 C12orf8 ERP28 Endoplasmic reticulum resident protein 29 Endoplasmic reticulum resident protein 28 Endoplasmic reticulum resident protein 31 |
| Molecular Weight(MW) | 28993 Da |
| Function | Does not seem to be a disulfide isomerase. Plays an important role in the processing of secretory proteins within the endoplasmic reticulum (ER), possibly by participating in the folding of proteins in the ER |
| Tissue Specificity | Ubiquitous. Mostly expressed in secretory tissues |
| Cellular Localization | Endoplasmic reticulum lumen. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV |
| Entrez Gene | 10961 |
|---|
Application
Western blot analysis of ERP29 Antibody (Center) in A2058,A375,MCF7,NCI-H460,Y79 cell line lysates (35ug/lane). ERP29 (arrow) was detected using the purified Pab.
Application
Western blot analysis of ERP29 (arrow) using rabbit polyclonal ERP29 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ERP29 gene (Lane 2) .
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ERP29 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
ERP29 Antibody (Center) flow cytometric analysis of NCI-H292 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
