Application
ERP44 Antibody (Center) western blot analysis in K562,NCI-H460,HL-60,MDA-MB435,CEM cell line lysates (35ug/lane).This demonstrates the ERP44 antibody detected the ERP44 protein (arrow).Application
ERP44 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ERP44 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
ERP44 Antibody (Center) flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ERP44 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA0573, TXNDC4 |
|---|---|
| Alternative Names | ERP44 KIAA0573 TXNDC4 Endoplasmic reticulum resident protein 44 Thioredoxin domain-containing protein 4 |
| Molecular Weight(MW) | 46971 Da |
| Function | Mediates thiol-dependent retention in the early secretory pathway, forming mixed disulfides with substrate proteins through its conserved CRFS motif. Inhibits the calcium channel activity of ITPR1. May have a role in the control of oxidative protein folding in the endoplasmic reticulum. Required to retain ERO1L and ERO1LB in the endoplasmic reticulum |
| Tissue Specificity | This ERP44 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 112~142 amino acids from the Central region of human ERP44. |
| Cellular Localization | Endoplasmic reticulum lumen. |
| Entrez Gene | 23071 |
|---|
Application
ERP44 Antibody (Center) western blot analysis in K562,NCI-H460,HL-60,MDA-MB435,CEM cell line lysates (35ug/lane).This demonstrates the ERP44 antibody detected the ERP44 protein (arrow).
Application
ERP44 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ERP44 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
ERP44 Antibody (Center) flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
