Application
Western blot analysis of ETV4 antibody (C-term) in mouse heart tissue lysates (35ug/lane). ETV4 (arrow) was detected using the purified Pab.Application
Western blot analysis of ETV4 (arrow) using rabbit polyclonal ETV4 Antibody (C-term) (RB20008). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ETV4 gene (Lane 2) .Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with ETV4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of widr cells using ETV4 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ETV4 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ETV4; E1AF; PEA3; ETS translocation variant 4; Adenovirus E1A enhancer-binding protein; E1A-F; Polyomavirus enhancer activator 3 homolog |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | E1AF PEA3 |
|---|---|
| Alternative Names | ETV4 E1AF PEA3 ETS translocation variant 4 Adenovirus E1A enhancer-binding protein E1A-F Polyomavirus enhancer activator 3 homolog |
| Molecular Weight(MW) | 53938 Da |
| Function | Transcriptional activator that binds to the enhancer of the adenovirus E1A gene; the core-binding sequence is 5'[AC]GGA[AT]GT-3' |
| Tissue Specificity | This ETV4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 426~455 amino acids from the C-terminal region of human ETV4. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 2118 |
|---|
Application
Western blot analysis of ETV4 antibody (C-term) in mouse heart tissue lysates (35ug/lane). ETV4 (arrow) was detected using the purified Pab.
Application
Western blot analysis of ETV4 (arrow) using rabbit polyclonal ETV4 Antibody (C-term) (RB20008). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ETV4 gene (Lane 2) .
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with ETV4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of widr cells using ETV4 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
