Application
Western blot analysis of anti-EXO1 Antibody (N-term) in NCI-H460 cell line lysates (35ug/lane). EXO1 (arrow) was detected using the purified Pab.Application
Confocal immunofluorescent analysis of EXO1 Antibody (N-term) with A549 cell followed by Alexa Fluor® 489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | EXO1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EXO1; EXOI; HEX1; Exonuclease 1; Exonuclease I |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:50~100 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | EXOI HEX1 |
|---|---|
| Alternative Names | EXO1 EXOI HEX1 Exonuclease 1 Exonuclease I |
| Molecular Weight(MW) | 94103 Da |
| Function | 5'->3' double-stranded DNA exonuclease which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity Functions in DNA mismatch repair (MMR) to excise mismatch- containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. Also exhibits endonuclease activity against 5'-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Required for somatic hypermutation (SHM) and class switch recombination (CSR) of immunoglobulin genes. Essential for male and female meiosis |
| Tissue Specificity | Highly expressed in bone marrow, testis and thymus. Expressed at lower levels in colon, lymph nodes, ovary, placenta, prostate, small intestine, spleen and stomach |
| Cellular Localization | Nucleus. Note=Colocalizes with PCNA to discrete nuclear foci in S-phase |
| Entrez Gene | 9156 |
|---|
Application
Western blot analysis of anti-EXO1 Antibody (N-term) in NCI-H460 cell line lysates (35ug/lane). EXO1 (arrow) was detected using the purified Pab.
Application
Confocal immunofluorescent analysis of EXO1 Antibody (N-term) with A549 cell followed by Alexa Fluor® 489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:50~100 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
