Application
EYA1 Antibody (N-term) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the EYA1 antibody detected the EYA1 protein (arrow).| Product Name | EYA1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EYA1; Eyes absent homolog 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | EYA1 Eyes absent homolog 1 |
|---|---|
| Molecular Weight(MW) | 64593 Da |
| Function | Tyrosine phosphatase that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph). 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1. Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. Seems to coactivate SIX2, SIX4 and SIX5. May be required for normal development of branchial arches, ear and kidney |
| Tissue Specificity | In the embryo, highly expressed in kidney with lower levels in brain. Weakly expressed in lung. In the adult, highly expressed in heart and skeletal muscle. Weakly expressed in brain and liver. No expression in eye or kidney |
| Cellular Localization | Cytoplasm (By similarity). Nucleus. Note=Localizes at sites of DNA damage at double-strand breaks (DSBs) |
| Entrez Gene | 2138 |
|---|---|
| Nucleotide Accession | NP_000494.2 NP_742057.1 NP_742055.1 |
Application
EYA1 Antibody (N-term) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the EYA1 antibody detected the EYA1 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
