Application
EYA2 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the EYA2 antibody detected the EYA2 protein (arrow).Application
Western blot analysis of EYA2 (arrow) using rabbit polyclonal EYA2 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the EYA2 gene.| Product Name | EYA2 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EYA2; EAB1; Eyes absent homolog 2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | EAB1 |
|---|---|
| Alternative Names | EYA2 EAB1 Eyes absent homolog 2 |
| Molecular Weight(MW) | 59232 Da |
| Function | Tyrosine phosphatase that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph). 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1. Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. Coactivates SIX1. Seems to coactivate SIX2, SIX4 and SIX5. Together with SIX1 and DACH2 seem to be involved in myogenesis. May be involved in development of the eye. Interaction with GNAZ and GNAI2 prevents nuclear translocation and transcriptional activity |
| Tissue Specificity | Highest expression in muscle with lower levels in kidney, placenta, pancreas, brain and heart |
| Cellular Localization | Cytoplasm. Nucleus. |
| Entrez Gene | 2139 |
|---|---|
| Nucleotide Accession | NP_742108.2 |
Application
EYA2 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the EYA2 antibody detected the EYA2 protein (arrow).
Application
Western blot analysis of EYA2 (arrow) using rabbit polyclonal EYA2 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the EYA2 gene.| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
