Application
A. Western blot analysis on (A) A431 and (B) MCF7 cell lysates using anti- Ezrin/Radixin/Moesin RabMAb (Cat. #AJ1279d), dilution 1:1000.Application
B. Immunohistochemical analysis of paraffin-embedded human breast adenocarcinoma using anti-EzrinRadixinMoesin RabMAb.Application
C. Immunofluorescent staining of HeLa cells using anti-EzrinRadixinMoesin (Cat. #AJ1279d).| Product Name | Ezrin/Radixin/Moesin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EZR, VIL2, Ezrin, p81;Cytovillin;Villin-2 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP2122Y |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:1000~5000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | VIL2 |
|---|---|
| Alternative Names | EZR VIL2 Ezrin p81 Cytovillin Villin-2 |
| Molecular Weight(MW) | 69413 Da |
| Function | Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis |
| Tissue Specificity | Expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. Weakly expressed in brain stem and diencephalon. Stronger expression was detected in gray matter of frontal lobe compared to white matter (at protein level). Component of the microvilli of intestinal epithelial cells. Preferentially expressed in astrocytes of hippocampus, frontal cortex, thalamus, parahippocampal cortex, amygdala, insula, and corpus callosum. Not detected in neurons in most tissues studied |
| Cellular Localization | Apical cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection. Cell projection, microvillus membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cell cortex. Cytoplasm, cytoskeleton. Note=Localization to the apical membrane of parietal cells depends on the interaction with MPP5. Localizes to cell extensions and peripheral processes of astrocytes (By similarity) Microvillar peripheral membrane protein (cytoplasmic side) |
| Entrez Gene | 7430 |
|---|
Application
A. Western blot analysis on (A) A431 and (B) MCF7 cell lysates using anti- Ezrin/Radixin/Moesin RabMAb (Cat. #AJ1279d), dilution 1:1000.
Application
B. Immunohistochemical analysis of paraffin-embedded human breast adenocarcinoma using anti-EzrinRadixinMoesin RabMAb.
Application
C. Immunofluorescent staining of HeLa cells using anti-EzrinRadixinMoesin (Cat. #AJ1279d).| Application Notes | WB:1:1000~5000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
