Application
A. Western blot analysis on (A) Jurkat, (B) 293T and (C) A375 cell lysates using anti-FIH1 RabMAb (Cat. #AJ1280a).Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-FIH1 RabMAb (Cat. #AJ1280a).Application
C. Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-FIH1 RabMAb (Cat. #AJ1280a).Application
D. Immunofluorescent staining of HeLa cells using anti-FIH1 RabMAb (Cat. #AJ1280a).| Product Name | F1H1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HIF1AN, FIH1, Hypoxia-inducible factor 1-alpha inhibitor, Hypoxia-inducible factor asparagine hydroxylase;Factor inhibiting HIF-1 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR3658 |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:5000~20000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | FIH1 |
|---|---|
| Alternative Names | HIF1AN FIH1 Hypoxia-inducible factor 1-alpha inhibitor Hypoxia-inducible factor asparagine hydroxylase Factor inhibiting HIF-1 |
| Molecular Weight(MW) | 40285 Da |
| Function | Hydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300- interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation |
| Tissue Specificity | A synthetic peptide corresponding to residues in human FIH1 was used as an immunogen. |
| Cellular Localization | Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Note=Mainly cytoplasmic localization, but interaction with NOTCH1 results in nuclear localization and interaction with ABPA3 results in perinuclear localization in macrophages |
| Entrez Gene | 55662 |
|---|
Application
A. Western blot analysis on (A) Jurkat, (B) 293T and (C) A375 cell lysates using anti-FIH1 RabMAb (Cat. #AJ1280a).
Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-FIH1 RabMAb (Cat. #AJ1280a).
Application
C. Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-FIH1 RabMAb (Cat. #AJ1280a).
Application
D. Immunofluorescent staining of HeLa cells using anti-FIH1 RabMAb (Cat. #AJ1280a).| Application Notes | WB:1:5000~20000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
