Application
Western blot analysis of anti-HIF1AN (N-term) Pab in 293, HL60 and Jurkat cell line lysates. HIF1AN (N-term)(arrow) was detected using the purified Pab.| Product Name | HIF1AN Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:250~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | FIH1 |
|---|---|
| Alternative Names | HIF1AN FIH1 Hypoxia-inducible factor 1-alpha inhibitor Factor inhibiting HIF-1 Hypoxia-inducible factor asparagine hydroxylase |
| Molecular Weight(MW) | 40285 Da |
| Function | Hydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300- interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation |
| Tissue Specificity | This HIF1AN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 31~61 amino acids from the N-terminal region of human HIF1AN. |
| Cellular Localization | Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Note=Mainly cytoplasmic localization, but interaction with NOTCH1 results in nuclear localization and interaction with ABPA3 results in perinuclear localization in macrophages |
| Entrez Gene | 55662 |
|---|
Application
Western blot analysis of anti-HIF1AN (N-term) Pab in 293, HL60 and Jurkat cell line lysates. HIF1AN (N-term)(arrow) was detected using the purified Pab.| Application Notes | WB:1:250~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
