Application
Western blot analysis of FA96B Antibody (N-term) in A375 cell line lysates (35ug/lane). FA96B (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human skin reacted with FA96B Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
FA96B Antibody (N-term) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | FA96B Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FAM96B; MIP18; Mitotic spindle-associated MMXD complex subunit MIP18; MSS19-interacting protein of 18 kDa; Protein FAM96B |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MIP18 |
|---|---|
| Alternative Names | FAM96B MIP18 Mitotic spindle-associated MMXD complex subunit MIP18 MSS19-interacting protein of 18 kDa Protein FAM96B |
| Molecular Weight(MW) | 17663 Da |
| Function | Component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into extramitochondrial Fe/S proteins. As part of the mitotic spindle-associated MMXD complex it plays a role in chromosome segregation, probably by facilitating iron-sulfur cluster assembly into ERCC2/XPD |
| Tissue Specificity | This FA96B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 23~53 amino acids from the C-terminal region of human WDR75. |
| Cellular Localization | Nucleus. Cytoplasm, cytoskeleton, spindle. |
| Entrez Gene | 51647 |
|---|
Application
Western blot analysis of FA96B Antibody (N-term) in A375 cell line lysates (35ug/lane). FA96B (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human skin reacted with FA96B Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
FA96B Antibody (N-term) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
