Application
Western blot analysis of Factor XIIIa Antibody (C-term) in K562 cell line lysates (35ug/lane). Factor XIIIa (arrow) was detected using the purified Pab.Application
Western blot analysis of F13A1 (arrow) using rabbit polyclonal Factor XIIIa Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the F13A1 gene (Lane 2).Application
Factor XIIIa Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Factor XIIIa Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Factor XIIIa Antibody (C-term) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | Factor XIIIa Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | F13A1; F13A; Coagulation factor XIII A chain; Protein-glutamine gamma-glutamyltransferase A chain; Transglutaminase A chain |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | F13A |
|---|---|
| Alternative Names | F13A1 F13A Coagulation factor XIII A chain Protein-glutamine gamma-glutamyltransferase A chain Transglutaminase A chain |
| Molecular Weight(MW) | 83267 Da |
| Function | Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl- epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin |
| Tissue Specificity | This Factor XIIIa antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 554~584 amino acids from the C-terminal region of human Factor XIIIa. |
| Cellular Localization | Cytoplasm. Secreted. Note=Secreted into the blood plasma. Cytoplasmic in most tissues, but also secreted in the blood plasma |
| Entrez Gene | 2162 |
|---|
Application
Western blot analysis of Factor XIIIa Antibody (C-term) in K562 cell line lysates (35ug/lane). Factor XIIIa (arrow) was detected using the purified Pab.
Application
Western blot analysis of F13A1 (arrow) using rabbit polyclonal Factor XIIIa Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the F13A1 gene (Lane 2).
Application
Factor XIIIa Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Factor XIIIa Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Factor XIIIa Antibody (C-term) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
