Application
A. Western blot analysis on Jurkat cell lysates using anti-phosho FADD (pS194) RabMAb (Cat. #AJ1284b), 1:500 dilution. Cells were either (A) untreated (B) treated with calyculin A| Product Name | FADD Antibody Phospho (pS194) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Fadd, Mort1, Protein FADD, FAS-associated death domain protein;FAS-associating death domain-containing protein;Mediator of receptor induced toxicity |
| Modification | p-S194 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR1816Y |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Mort1 |
|---|---|
| Alternative Names | Fadd Mort1 Protein FADD FAS-associated death domain protein FAS-associating death domain-containing protein Mediator of receptor induced toxicity |
| Molecular Weight(MW) | 22960 Da |
| Function | Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis (By similarity). Involved in interferon-mediated antiviral immune response, playing a role in the positive regulation of interferon signaling (By similarity) |
| Tissue Specificity | A synthetic peptide corresponding to residues surrounding serine 194 of human FADD was used as immunogen. |
| Entrez Gene | 14082 |
|---|
Application
A. Western blot analysis on Jurkat cell lysates using anti-phosho FADD (pS194) RabMAb (Cat. #AJ1284b), 1:500 dilution. Cells were either (A) untreated (B) treated with calyculin A| Application Notes | WB:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
