Application
Western blot analysis of IFITM3 (arrow) using rabbit polyclonal IFITM3 Antibody (C-term) (Cat# AP1153c).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the IFITM3 gene (Lane 2) (Origene Technologies).Application
Western blot analysis of anti-IFITM3 Antibody (Center) (Cat# AP1153c) in Hela cell line lysates (35ug/lane). IFITM3(arrow) was detected using the purified Pab (1:60 dilution).| Product Name | Fagilis (IFITM3) Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IFITM3; Interferon-induced transmembrane protein 3; Dispanin subfamily A member 2b; Interferon-inducible protein 1-8U |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:50~100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | IFITM3 Interferon-induced transmembrane protein 3 Dispanin subfamily A member 2b Interferon-inducible protein 1-8U |
|---|---|
| Molecular Weight(MW) | 14632 Da |
| Function | IFN-induced antiviral protein which inhibits the entry of viruses to the host cell cytoplasm, permitting endocytosis, but preventing subsequent viral fusion and release of viral contents into the cytosol. Active against multiple viruses, including influenza A virus, SARS coronavirus (SARS-CoV), Marburg virus (MARV) and Ebola virus (EBOV), Dengue virus (DNV), West Nile virus (WNV), human immunodeficiency virus type 1 (HIV-1) and vesicular stomatitis virus (VSV). Can inhibit: influenza virus hemagglutinin protein-mediated viral entry, MARV and EBOV GP1,2-mediated viral entry, SARS-CoV S protein-mediated viral entry and VSV G protein- mediated viral entry. Plays a critical role in the structural stability and function of vacuolar ATPase (v-ATPase). Establishes physical contact with the v-ATPase of endosomes which is critical for proper clathrin localization and is also required for the function of the v-ATPase to lower the pH in phagocytic endosomes thus establishing an antiviral state |
| Tissue Specificity | This IFITM3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 71~101 amino acids from the Center region of human IFITM3. |
| Cellular Localization | Cell membrane; Single-pass type II membrane protein. Late endosome membrane; Single-pass type II membrane protein. Lysosome membrane; Single-pass type II membrane protein |
| Entrez Gene | 10410 |
|---|
Application
Western blot analysis of IFITM3 (arrow) using rabbit polyclonal IFITM3 Antibody (C-term) (Cat# AP1153c).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the IFITM3 gene (Lane 2) (Origene Technologies).
Application
Western blot analysis of anti-IFITM3 Antibody (Center) (Cat# AP1153c) in Hela cell line lysates (35ug/lane). IFITM3(arrow) was detected using the purified Pab (1:60 dilution).| Application Notes | WB:1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
