Application
Fluorescent image of U251 cells stained with AP19452c FAM176A (Center) antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP19452c FAM176A (Center) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). FAM176A immunoreactivity is localized to the cytoplasmic vesicles of U251 cells.Application
FAM176A Antibody (Center) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the FAM176A antibody detected the FAM176A protein (arrow).| Product Name | FAM176A (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FAM176A; TMEM166; Protein FAM176A; Transmembrane protein 166 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFWB |
| IF:1:200 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | FAM176A TMEM166 |
|---|---|
| Alternative Names | FAM176A TMEM166 Protein FAM176A Transmembrane protein 166 |
| Molecular Weight(MW) | 17470 Da |
| Function | Acts as a regulator of programmed cell death, mediating both autophagy and apoptosis |
| Tissue Specificity | Expressed in lung, kidney, liver, pancreas, placenta, but not in heart and skeletal muscle |
| Cellular Localization | Endoplasmic reticulum membrane; Single-pass membrane protein. Lysosome membrane; Single-pass membrane protein |
| Entrez Gene | 84141 |
|---|---|
| Nucleotide Accession | NP_115557.1 |
Application
Fluorescent image of U251 cells stained with AP19452c FAM176A (Center) antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP19452c FAM176A (Center) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). FAM176A immunoreactivity is localized to the cytoplasmic vesicles of U251 cells.
Application
FAM176A Antibody (Center) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the FAM176A antibody detected the FAM176A protein (arrow).| Application Notes | IF:1:200 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
