Application
Western blot analysis of FBL Antibody (Center P212) in Hela cell line lysates (35ug/lane). FBL (arrow) was detected using the purified Pab.Application
FBL Antibody (Center P212) flow cytometric analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | FBL Antibody (Center P212) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FBL; FIB1; FLRN; rRNA 2'-O-methyltransferase fibrillarin; 34 kDa nucleolar scleroderma antigen |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:50~100 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | FIB1 FLRN |
|---|---|
| Alternative Names | FBL FIB1 FLRN rRNA 2'-O-methyltransferase fibrillarin 34 kDa nucleolar scleroderma antigen |
| Molecular Weight(MW) | 33784 Da |
| Function | Involved in pre-rRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'- hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA |
| Tissue Specificity | This FBL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 203~233 amino acids from the Center (P212) region of human FBL. |
| Cellular Localization | Nucleus, nucleolus. Note=Fibrillar region of the nucleolus |
| Entrez Gene | 2091 |
|---|
Application
Western blot analysis of FBL Antibody (Center P212) in Hela cell line lysates (35ug/lane). FBL (arrow) was detected using the purified Pab.
Application
FBL Antibody (Center P212) flow cytometric analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
