Application
FBLIM1 Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the FBLIM1 antibody detected the FBLIM1 protein (arrow).Application
FBLIM1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FBLIM1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | FBLIM1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FBLIM1; FBLP1; Filamin-binding LIM protein 1; Migfilin; Mitogen-inducible 2-interacting protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | FBLP1 |
|---|---|
| Alternative Names | FBLIM1 FBLP1 Filamin-binding LIM protein 1 Migfilin Mitogen-inducible 2-interacting protein |
| Molecular Weight(MW) | 40670 Da |
| Function | Serves as an anchoring site for cell-ECM adhesion proteins and filamin-containing actin filaments. Is implicated in cell shape modulation (spreading) and motility. May participate in the regulation of filamin-mediated cross-linking and stabilization of actin filaments. May also regulate the assembly of filamin- containing signaling complexes that control actin assembly Promotes dissociation of FLNA from ITGB3 and ITGB7. Promotes activation of integrins and regulates integrin-mediated cell-cell adhesion |
| Tissue Specificity | Isoform 1 and isoform 3 are expressed in heart, kidney, lung, pancreas, placenta and platelets. Isoform 2 is expressed in brain, heart, kidney, lung, pancreas, placenta, skeletal muscle and platelets |
| Cellular Localization | Cytoplasm, cell cortex. Cell junction, focal adhesion. Cytoplasm, cytoskeleton. Note=Associated with actin stress fiber at cell-ECM focal adhesion sites. Isoform 1 and isoform 3 are recruited and localized at actin stress fibers and clustered at cell-EMC adhesion sites through interaction with PLEKHC1. Isoform 2 is localized at actin stress fibers |
| Entrez Gene | 54751 |
|---|---|
| Nucleotide Accession | NP_001019386.1 NP_060026.2 |
Application
FBLIM1 Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the FBLIM1 antibody detected the FBLIM1 protein (arrow).
Application
FBLIM1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FBLIM1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
