Application
Western blot analysis of FBXL2 Antibody (C-term) in MDA-MB435 cell line lysates (35ug/lane).FBXL2 (arrow) was detected using the purified Pab.Application
FBXL2 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FBXL2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
FBXL2 Antibody (C-term) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | FBXL2 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | FBL2, FBL3 |
|---|---|
| Alternative Names | FBXL2 FBL2 FBL3 F-box/LRR-repeat protein 2 F-box and leucine-rich repeat protein 2 F-box protein FBL2/FBL3 |
| Molecular Weight(MW) | 47062 Da |
| Function | Calcium-activated substrate recognition component of a SCF (SKP1-cullin-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Unlike many F-box proteins, FBXL2 doesn't seem to target phosphodegron within its substrates but rather calmodulin-binding motifs. Targets PCYT1A for its monoubiquitination and degradation, this is antagonized by calmodulin (By similarity). Targets the cyclins CCND2 and CCND3 for polyubiquitination and degradation, leading to cell-cycle arrest in G(0), also antagonized by calmodulin. Binds to hepatitis C virus non-structural protein 5A (NS5A) in a reaction crucial for hepatitis C virus RNA replication |
| Tissue Specificity | Expressed in brain, heart, kidney, liver, lung, pancreas and placenta |
| Cellular Localization | Membrane; Lipid-anchor. |
| Entrez Gene | 25827 |
|---|
Application
Western blot analysis of FBXL2 Antibody (C-term) in MDA-MB435 cell line lysates (35ug/lane).FBXL2 (arrow) was detected using the purified Pab.
Application
FBXL2 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FBXL2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
FBXL2 Antibody (C-term) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
