Application
Western blot analysis of FBXO3 Antibody (C-term) in HepG2 cell line lysates (35ug/lane). FBXO3 (arrow) was detected using the purified Pab.Application
FBXO3 Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | FBXO3 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | FBX3 |
|---|---|
| Alternative Names | FBXO3 FBX3 F-box only protein 3 |
| Molecular Weight(MW) | 54561 Da |
| Function | Substrate recognition component of the SCF (SKP1-CUL1-F- box protein)-type E3 ubiquitin ligase complex. Mediates the ubiquitination of HIPK2 and probably that of EP300, leading to rapid degradation by the proteasome. In the presence of PML, HIPK2 ubiquitination still occurs, but degradation is prevented. PML, HIPK2 and FBXO3 may act synergically to activate p53/TP53- dependent transactivation |
| Tissue Specificity | This FBXO3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 401~431 amino acids from the C-terminal region of human FBXO3. |
| Cellular Localization | Nucleus. Note=Colocalizes with PML at the peripheries of nuclear bodies |
| Entrez Gene | 26273 |
|---|
Application
Western blot analysis of FBXO3 Antibody (C-term) in HepG2 cell line lysates (35ug/lane). FBXO3 (arrow) was detected using the purified Pab.
Application
FBXO3 Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
