Application
FBXO5 Antibody (Center) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the FBXO5 antibody detected the FBXO5 protein (arrow).| Product Name | FBXO5 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | EMI1, FBX5 |
|---|---|
| Alternative Names | FBXO5 EMI1 FBX5 F-box only protein 5 Early mitotic inhibitor 1 |
| Molecular Weight(MW) | 50146 Da |
| Function | Regulates progression through early mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Binds to the APC activators CDC20 and FZR1/CDH1 to prevent APC activation. Can also bind directly to the APC to inhibit substrate-binding |
| Tissue Specificity | This FBXO5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 191-221 amino acids from the Central region of human FBXO5. |
| Cellular Localization | Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle. Note=In interphase, localizes in a punctate manner in the nucleus and cytoplasm with some perinuclear concentration. In mitotic cells, localizes throughout the cell, particularly at the spindle |
| Entrez Gene | 26271 |
|---|---|
| Nucleotide Accession | NP_036309.1 NP_001135994.1 |
Application
FBXO5 Antibody (Center) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the FBXO5 antibody detected the FBXO5 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
