Application
FBXW5 Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the FBXW5 antibody detected the FBXW5 protein (arrow).| Product Name | FBXW5 (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | FBW5 |
|---|---|
| Alternative Names | FBXW5 FBW5 F-box/WD repeat-containing protein 5 F-box and WD-40 domain-containing protein 5 |
| Molecular Weight(MW) | 63922 Da |
| Function | Substrate recognition component of both SCF (SKP1-CUL1- F-box protein) and DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes. Substrate recognition component of the SCF(FBXW5) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of SASS6 during S phase, leading to prevent centriole reduplication. The SCF(FBXW5) complex also mediates ubiquitination and degradation of actin-regulator EPS8 during G2 phase, leading to the transient degradation of EPS8 and subsequent cell shape changes required to allow mitotic progression. Substrate-specific adapter of the DCX(FBXW5) E3 ubiquitin-protein ligase complex which mediates the polyubiquitination and subsequent degradation of TSC2. May also act as a negative regulator of MAP3K7/TAK1 signaling in the interleukin-1B (IL1B) signaling pathway |
| Tissue Specificity | This FBXW5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 324-350 amino acids from the Central region of human FBXW5. |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 54461 |
|---|---|
| Nucleotide Accession | NP_061871.1 |
Application
FBXW5 Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the FBXW5 antibody detected the FBXW5 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
