Application
FBXO9 Antibody (C-term) western blot analysis in mouse liver tissue lysates (15ug/lane).This demonstrates the FBXO9 antibody detected FBXO9 protein (arrow).Application
FBXO9 Antibody (C-term) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | FBXO9 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | FBX9, VCIA1 |
|---|---|
| Alternative Names | FBXO9 FBX9 VCIA1 F-box only protein 9 Cross-immune reaction antigen 1 Renal carcinoma antigen NY-REN-57 |
| Molecular Weight(MW) | 52329 Da |
| Function | Substrate recognition component of a (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of TTI1 and TELO2 in a CK2-dependent manner, thereby directly regulating mTOR signaling. SCF(FBXO9) recognizes and binds mTORC1-bound TTI1 and TELO2 when they are phosphorylated by CK2 following growth factor deprivation, leading to their degradation. In contrast, the SCF(FBXO9) does not mediate ubiquitination of TTI1 and TELO2 when they are part of the mTORC2 complex. As a consequence, mTORC1 is inactivated to restrain cell growth and protein translation, while mTORC2 is activated due to the relief of feedback inhibition by mTORC1 |
| Tissue Specificity | This FBXO9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 353-382 amino acids from the C-terminal region of human FBXO9. |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 26268 |
|---|---|
| Nucleotide Accession | NP_036479.1 |
Application
FBXO9 Antibody (C-term) western blot analysis in mouse liver tissue lysates (15ug/lane).This demonstrates the FBXO9 antibody detected FBXO9 protein (arrow).
Application
FBXO9 Antibody (C-term) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
