Application
FBXW11 Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the FBXW11 antibody detected the FBXW11 protein (arrow).Application
FBXW11 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FBXW11 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
FBXW11 Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | FBXW11 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BTRCP2, FBW1B, FBXW1B, KIAA0696 |
|---|---|
| Alternative Names | FBXW11 BTRCP2 FBW1B FBXW1B KIAA0696 F-box/WD repeat-containing protein 11 F-box and WD repeats protein beta-TrCP2 F-box/WD repeat-containing protein 1B Homologous to Slimb protein |
| Molecular Weight(MW) | 62091 Da |
| Function | Substrate recognition component of a SCF (SKP1-CUL1-F- box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins. SCF(FBXW11) mediates the ubiquitination of CTNNB1 and participates in Wnt signaling. SCF(FBXW11) mediates the ubiquitination of phosphorylated NFKBIA, which degradation frees the associated NFKB1 to translocate into the nucleus and to activate transcription. SCF(FBXW11) mediates the ubiquitination of IFNAR1. Targets phosphorylation-dependent degradation of beta- catenin. Involved in the oxidative stress-induced a ubiquitin- mediated decrease in RCAN1. Mediates the degradation of CDC25A induced by ionizing radiation in cells progressing through S phase and thus may function in the intra-S-phase checkpoint. Has an essential role in the control of the clock-dependent transcription via degradation of PER1 and phosphorylated PER2. Is target of human immunodeficiency virus type 1 (HIV-1) protein VPU to polyubiquitinate and deplete BST2 from cells and antagonize its antiviral action |
| Tissue Specificity | This FBXW11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 175-204 amino acids from the Central region of human FBXW11. |
| Cellular Localization | Cytoplasm (By similarity). Nucleus (By similarity) |
Application
FBXW11 Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the FBXW11 antibody detected the FBXW11 protein (arrow).
Application
FBXW11 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FBXW11 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
FBXW11 Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
