Application
FCHO2 Antibody (Center) western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the FCHO2 antibody detected the FCHO2 protein (arrow).| Product Name | FCHO2 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | FCHO2 FCH domain only protein 2 |
|---|---|
| Molecular Weight(MW) | 88924 Da |
| Function | Functions in an early step of clathrin-mediated endocytosis. Has both a membrane binding/bending activity and the ability to recruit proteins essential to the formation of functional clathrin-coated pits. Has a lipid-binding activity with a preference for membranes enriched in phosphatidylserine and phosphoinositides (Pi(4,5) biphosphate) like the plasma membrane Its membrane-bending activity might be important for the subsequent action of clathrin and adaptors in the formation of clathrin-coated vesicles. Involved in adaptor protein complex AP- 2-dependent endocytosis of the transferin receptor, it also functions in the AP-2-independent endocytosis of the LDL receptor |
| Tissue Specificity | This FCHO2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 136-165 amino acids from the Central region of human FCHO2. |
| Cellular Localization | Membrane, clathrin-coated pit; Peripheral membrane protein; Cytoplasmic side (By similarity) Note=Associated with forming but not mature clathrin-coated vesicles. The recruitment to coated-pits precede the one of clathrin and the adaptor protein complex AP-2 (By similarity) |
| Entrez Gene | 115548 |
|---|---|
| Nucleotide Accession | NP_620137.2 |
Application
FCHO2 Antibody (Center) western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the FCHO2 antibody detected the FCHO2 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
