Application
Western blot analysis of anti-FEN1 Antibody (Center) in HepG2 cell line lysates (35ug/lane). FEN1(arrow) was detected using the purified Pab.Application
Western blot analysis of FEN1 (arrow) using rabbit polyclonal FEN1 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FEN1 gene.Application
Confocal immunofluorescent analysis of FEN1 Antibody (Center) with Hela cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | FEN1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:50~100 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | RAD2 |
|---|---|
| Alternative Names | FEN1 RAD2 Flap endonuclease 1 DNase IV Flap structure-specific endonuclease 1 Maturation factor 1 |
| Molecular Weight(MW) | 42593 Da |
| Function | Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double- stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA |
| Tissue Specificity | This FEN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 250~280 amino acids from the Center region of human FEN1. |
| Cellular Localization | Nucleus, nucleolus. Nucleus, nucleoplasm. Mitochondrion (By similarity). Note=Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage |
| Entrez Gene | 2237 |
|---|
Application
Western blot analysis of anti-FEN1 Antibody (Center) in HepG2 cell line lysates (35ug/lane). FEN1(arrow) was detected using the purified Pab.
Application
Western blot analysis of FEN1 (arrow) using rabbit polyclonal FEN1 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FEN1 gene.
Application
Confocal immunofluorescent analysis of FEN1 Antibody (Center) with Hela cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:50~100 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
