Application
Figure 1: Western blot analysis using anti-FGF2 monoclonal antibody against truncated FGF2 recombinant protein.Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human recturn adenocarcinoma tissue showing cytoplasmic localization using anti-FGF2 monoclonal antibody with DAB staining.| Product Name | FGF2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Clone Number | 2H5G2C11 |
| Host Species | Mouse |
| Tested Applications | WBIHC |
| WB:1:500 - 2000. IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | FGFB |
|---|---|
| Alternative Names | BFGF FGFB HBGF-2 FGF2 |
| Molecular Weight(MW) | 30770 Da |
| Function | Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro |
| Tissue Specificity | Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non- cancerous liver tissue |
| Cellular Localization | Secreted. Nucleus. Note=Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57 |
| Entrez Gene | 2247 |
|---|---|
| Nucleotide Accession | NP_001997.5 |
Application
Figure 1: Western blot analysis using anti-FGF2 monoclonal antibody against truncated FGF2 recombinant protein.
Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human recturn adenocarcinoma tissue showing cytoplasmic localization using anti-FGF2 monoclonal antibody with DAB staining.| Application Notes | WB:1:500 - 2000. IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of FGF2 expressed in E. Coli. |
