Application
Western blot analysis of FGFR4 (arrow) using rabbit polyclonal FGFR4 Antibody .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the FGFR4 gene (Lane 2) (Origene Technologies).| Product Name | FGFR4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | JTK2, TKF |
|---|---|
| Alternative Names | FGFR4 JTK2 TKF Fibroblast growth factor receptor 4 CD_antigen=CD334 Flags: Precursor |
| Molecular Weight(MW) | 87954 Da |
| Function | Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays a role in the regulation of cell proliferation, differentiation and migration, and in regulation of lipid metabolism, bile acid biosynthesis, glucose uptake, vitamin D metabolism and phosphate homeostasis Required for normal down-regulation of the expression of CYP7A1, the rate-limiting enzyme in bile acid synthesis, in response to FGF19. Phosphorylates PLCG1 and FRS2. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes SRC-dependent phosphorylation of the matrix protease MMP14 and its lysosomal degradation. FGFR4 signaling is down-regulated by receptor internalization and degradation; MMP14 promotes internalization and degradation of FGFR4. Mutations that lead to constitutive kinase activation or impair normal FGFR4 inactivation lead to aberrant signaling |
| Tissue Specificity | Expressed in gastrointestinal epithelial cells, pancreas, and gastric and pancreatic cancer cell lines |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. Endosome. Endoplasmic reticulum. Note=Internalized from the cell membrane to recycling endosomes, and from there back to the cell membrane |
| Entrez Gene | 2264 |
|---|
Application
Western blot analysis of FGFR4 (arrow) using rabbit polyclonal FGFR4 Antibody .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the FGFR4 gene (Lane 2) (Origene Technologies).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
