Application
Western blot analysis of FLI1 antibody (N-term) (Cat.# AP6514a) in 293 cell line lysates (35ug/lane). FLI1 (arrow) was detected using the purified Pab.Application
FLI1 Antibody (N-term) (Cat.# AP6514a) IHC analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FLI1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Western blot analysis of FLI1 (arrow) using rabbit polyclonal FLI1 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FLI1 gene.Application
FLI1 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | FLI1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FLI1; Friend leukemia integration 1 transcription factor; Proto-oncogene Fli-1; Transcription factor ERGB |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | FLI1 Friend leukemia integration 1 transcription factor Proto-oncogene Fli-1 Transcription factor ERGB |
|---|---|
| Molecular Weight(MW) | 50982 Da |
| Function | Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3' |
| Tissue Specificity | This FLI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 60~90 amino acids from the N-terminal region of human FLI1. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 2313 |
|---|
Application
Western blot analysis of FLI1 antibody (N-term) (Cat.# AP6514a) in 293 cell line lysates (35ug/lane). FLI1 (arrow) was detected using the purified Pab.
Application
FLI1 Antibody (N-term) (Cat.# AP6514a) IHC analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FLI1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Western blot analysis of FLI1 (arrow) using rabbit polyclonal FLI1 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FLI1 gene.
Application
FLI1 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
