Application
Figure 2: Western blot analysis using FOXA2 mouse mAb against A549 (1) cell lysate.Application
Figure 3: Immunohistochemical analysis of paraffin-embedded human colon cancer (A), gastric cancer (B) and rectal cancer (C)tissues using FOXA2 mouse antibody with DAB staining.| Product Name | FOXA2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HNF3B; TCF3B; MGC19807 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 7H4B7 |
| Host Species | Mouse |
| Tested Applications | WBIHC |
| WB:1:500 - 2000. IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HNF3B TCF3B |
|---|---|
| Alternative Names | HNF3B TCF3B MGC19807 |
| Molecular Weight(MW) | 48306 Da |
| Function | Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity) In embryonic development is required for notochord formation Involved in the development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs; FOXA1 and FOXA2 seem to have at least in part redundant roles. Originally described as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc Interacts with the cis-acting regulatory regions of these genes Involved in glucose homeostasis; regulates the expression of genes important for glucose sensing in pancreatic beta-cells and glucose homeostasis. Involved in regulation of fat metabolism. Binds to fibrinogen beta promoter and is involved in IL6-induced fibrinogen beta transcriptional activation |
| Tissue Specificity | Purified recombinant fragment of FOXA2 expressed in E. Coli. |
| Cellular Localization | Nucleus. Cytoplasm. Note=Shuttles between the nucleus and cytoplasm in a CRM1-dependent manner; in response to insulin signaling via AKT1 is exported from the nucleus |
| Entrez Gene | 3170 |
|---|---|
| Nucleotide Accession | NP_710141.1 |
Application
Figure 2: Western blot analysis using FOXA2 mouse mAb against A549 (1) cell lysate.
Application
Figure 3: Immunohistochemical analysis of paraffin-embedded human colon cancer (A), gastric cancer (B) and rectal cancer (C)tissues using FOXA2 mouse antibody with DAB staining.| Application Notes | WB:1:500 - 2000. IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of FOXA2 expressed in E. Coli. |
