Application
Western blot analysis of FOXG1 Antibody (Center) in mouse brain tissue lysates (35ug/lane). FOXG1 (arrow) was detected using the purified Pab.Application
FOXG1 Antibody (Center) flow cytometric analysis of U251 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Fluorescent confocal image of A549 cell stained with FOXG1 Antibody (Center)(Cat#AP9793c).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with FOXG1 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). FOXG1 immunoreactivity is localized to Nucleus significantly.| Product Name | FOXG1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FOXG1; FKH2; FKHL1; FKHL2; FKHL3; FKHL4; FOXG1A; FOXG1B; FOXG1C; Forkhead box protein G1; Brain factor 1; Brain factor 2; Forkhead box protein G1A; Forkhead box protein G1B; Forkhead box protein G1C; Forkhead-related protein FKHL1; Forkhead-related protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFCIF |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | FOXG1 FKH2 FKHL1 FKHL2 FKHL3 FKHL4 FOXG1A FOXG1B FOXG1C Forkhead box protein G1 Brain factor 1 Brain factor 2 Forkhead box protein G1A Forkhead box protein G1B Forkhead box protein G1C Forkhead-related protein FKHL1 Forkhead-related protein |
|---|---|
| Molecular Weight(MW) | 52352 Da |
| Function | Transcription repression factor which plays an important role in the establishment of the regional subdivision of the developing brain and in the development of the telencephalon |
| Tissue Specificity | Expression is restricted to the neurons of the developing telencephalon |
| Cellular Localization | Nucleus. |
| Entrez Gene | 2290 |
|---|
Application
Western blot analysis of FOXG1 Antibody (Center) in mouse brain tissue lysates (35ug/lane). FOXG1 (arrow) was detected using the purified Pab.
Application
FOXG1 Antibody (Center) flow cytometric analysis of U251 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Fluorescent confocal image of A549 cell stained with FOXG1 Antibody (Center)(Cat#AP9793c).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with FOXG1 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). FOXG1 immunoreactivity is localized to Nucleus significantly.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
