Application
Western blot analysis of FPGS Antibody (Center) in K562, A2058 cell line lysates (35ug/lane). FPGS (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human lung carcinoma with FPGS Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of K562 cells using FPGS Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | FPGS Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FPGS; Folylpolyglutamate synthase, mitochondrial; Folylpoly-gamma-glutamate synthetase; Tetrahydrofolylpolyglutamate synthase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | FPGS Folylpolyglutamate synthase mitochondrial Folylpoly-gamma-glutamate synthetase Tetrahydrofolylpolyglutamate synthase |
|---|---|
| Molecular Weight(MW) | 64609 Da |
| Function | Catalyzes conversion of folates to polyglutamate derivatives allowing concentration of folate compounds in the cell and the intracellular retention of these cofactors, which are important substrates for most of the folate-dependent enzymes that are involved in one-carbon transfer reactions involved in purine, pyrimidine and amino acid synthesis. Unsubstitued reduced folates are the preferred substrates. Metabolizes methotrexate (MTX) to polyglutamates |
| Tissue Specificity | This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 310~339 amino acids from the Center region of human FPGS. |
| Cellular Localization | Isoform 1: Mitochondrion inner membrane. Mitochondrion matrix |
| Entrez Gene | 2356 |
|---|
Application
Western blot analysis of FPGS Antibody (Center) in K562, A2058 cell line lysates (35ug/lane). FPGS (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human lung carcinoma with FPGS Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of K562 cells using FPGS Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
