Application
Western blot analysis of PFKM polyclonal antibody in Hela cell line lysate. PFKM(arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PFKM Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PFKM antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of Fructose 6 Phosphate Kinase (PFKM) Antibody(C-term)(Cat#AP8137b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | Fructose 6 Phosphate Kinase (PFKM) Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PFKM; PFKX; 6-phosphofructokinase, muscle type; Phosphofructo-1-kinase isozyme A; Phosphofructokinase 1; Phosphohexokinase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PFKX |
|---|---|
| Alternative Names | PFKM PFKX 6-phosphofructokinase muscle type Phosphofructo-1-kinase isozyme A Phosphofructokinase 1 Phosphohexokinase |
| Molecular Weight(MW) | 85183 Da |
| Function | Catalyzes the third step of glycolysis, the phosphorylation of fructose-6-phosphate (F6P) by ATP to generate fructose-1,6-bisphosphate (FBP) and ADP |
| Tissue Specificity | This PFKM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 751~780 amino acids from the C-terminal region of human PFKM. |
| Entrez Gene | 5213 |
|---|
Application
Western blot analysis of PFKM polyclonal antibody in Hela cell line lysate. PFKM(arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PFKM Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PFKM antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of Fructose 6 Phosphate Kinase (PFKM) Antibody(C-term)(Cat#AP8137b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
