Application
Western blot analysis of FUCA1 Antibody (Center) in HepG2 cell line lysates (35ug/lane). FUCA1 (arrow) was detected using the purified Pab.Application
FUCA1 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FUCA1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
FUCA1 Antibody (Center) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | FUCA1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | FUCA1 Tissue alpha-L-fucosidase Alpha-L-fucosidase I Alpha-L-fucoside fucohydrolase 1 |
|---|---|
| Molecular Weight(MW) | 53689 Da |
| Function | Alpha-L-fucosidase is responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N- acetylglucosamine of the carbohydrate moieties of glycoproteins |
| Tissue Specificity | This FUCA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 272~302 amino acids from the Center region of human FUCA1. |
| Cellular Localization | Lysosome. |
| Entrez Gene | 2517 |
|---|
Application
Western blot analysis of FUCA1 Antibody (Center) in HepG2 cell line lysates (35ug/lane). FUCA1 (arrow) was detected using the purified Pab.
Application
FUCA1 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FUCA1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
FUCA1 Antibody (Center) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
