Application
Western blot analysis of FZD4 Antibody (C-term) in 293 cell line lysates (35ug/lane).FZD4 (arrow) was detected using the purified Pab;Application
Western blot analysis of FZD4 Antibody (C-term) in mouse heart tissue lysates (35ug/lane). FZD4 (arrow) was detected using the purified Pab.| Product Name | FZD4 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | FZD4 Frizzled-4 FzE4 CD_antigen=CD344 Flags: Precursor |
|---|---|
| Molecular Weight(MW) | 59881 Da |
| Function | Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin (CTNNB1) canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin (CTNNB1) and activation of Wnt target genes. Plays a critical role in retinal vascularization by acting as a receptor for Wnt proteins and norrin (NDP). In retina, it can be both activated by Wnt protein-binding, but also by a Wnt-independent signaling via binding of norrin (NDP), promoting in both cases beta-catenin (CTNNB1) accumulation and stimulation of LEF/TCF-mediated transcriptional programs. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues |
| Tissue Specificity | Almost ubiquitous. Largely expressed in adult heart, skeletal muscle, ovary, and fetal kidney. Moderate amounts in adult liver, kidney, pancreas, spleen, and fetal lung, and small amounts in placenta, adult lung, prostate, testis, colon, fetal brain and liver |
| Cellular Localization | Membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein (By similarity) |
| Entrez Gene | 8322 |
|---|
Application
Western blot analysis of FZD4 Antibody (C-term) in 293 cell line lysates (35ug/lane).FZD4 (arrow) was detected using the purified Pab;
Application
Western blot analysis of FZD4 Antibody (C-term) in mouse heart tissue lysates (35ug/lane). FZD4 (arrow) was detected using the purified Pab.| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
