Application
FZR1 Antibody (N-term) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the FZR1 antibody detected the FZR1 protein (arrow).| Product Name | FZR1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CDH1, FYR, FZR, KIAA1242 |
|---|---|
| Alternative Names | FZR1 CDH1 FYR FZR KIAA1242 Fizzy-related protein homolog CDC20-like protein 1 Cdh1/Hct1 homolog |
| Molecular Weight(MW) | 55179 Da |
| Function | Key regulator of ligase activity of the anaphase promoting complex/cyclosome (APC/C), which confers substrate specificity upon the complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis |
| Tissue Specificity | Isoform 2 is expressed at high levels in heart, liver, spleen and some cancer cell lines whereas isoform 3 is expressed only at low levels in these tissues |
| Cellular Localization | Isoform 2: Nucleus. |
| Entrez Gene | 51343 |
|---|---|
| Nucleotide Accession | NP_057347.2 |
Application
FZR1 Antibody (N-term) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the FZR1 antibody detected the FZR1 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
