Application
Fluorescent image of U251 cells stained with GABARAP (N-term) antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1821a GABARAP (N-term) primary antibody (1:500, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). GABARAP immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.Application
Western blot analysis of GABARAP (arrow) using purified Pab . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GABARAP gene (Lane 2) (Origene Technologies).Application
Rabbit anti-GABARAP Abgent AP1821a 1:75 dilution in 5% scimmed milk powder. Secondary antibody goat anti-rabbit-HRP ECL detection 30 sec on X-ray film.Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Autophagy GABARAP Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
GABARAP antibody was tested in mouse MEFs and mouse motor neurons. Cells were fixed in 3% PFA and permeabilized with 100% methanol. Antibodies used were Abgent anti-GABARAP (1:500, labeled with anti-rabbit 488 Alexa) and Covance anti-Tuj1 (1:1000, labeled with anti-mouse 647).| Product Name | GABARAP Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFWBIHC |
| IF:1:10~50 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | FLC3B |
|---|---|
| Alternative Names | GABARAP FLC3B Gamma-aminobutyric acid receptor-associated protein GABA(A) receptor-associated protein MM46 |
| Molecular Weight(MW) | 13918 Da |
| Function | May play a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Involved in apoptosis. Involved in autophagy (By similarity) |
| Tissue Specificity | Heart, brain, placenta, liver, skeletal muscle, kidney and pancreas |
| Cellular Localization | Endomembrane system (By similarity). Cytoplasm, cytoskeleton (By similarity). Golgi apparatus membrane (By similarity). Cytoplasmic vesicle, autophagosome. Note=Largely associated with intracellular membrane structures including the Golgi apparatus and postsynaptic cisternae. Colocalizes with microtubules (By similarity) |
| Entrez Gene | 11337 |
|---|
Application
Fluorescent image of U251 cells stained with GABARAP (N-term) antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1821a GABARAP (N-term) primary antibody (1:500, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). GABARAP immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
Application
Western blot analysis of GABARAP (arrow) using purified Pab . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GABARAP gene (Lane 2) (Origene Technologies).
Application
Rabbit anti-GABARAP Abgent AP1821a 1:75 dilution in 5% scimmed milk powder. Secondary antibody goat anti-rabbit-HRP ECL detection 30 sec on X-ray film.
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Autophagy GABARAP Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
GABARAP antibody was tested in mouse MEFs and mouse motor neurons. Cells were fixed in 3% PFA and permeabilized with 100% methanol. Antibodies used were Abgent anti-GABARAP (1:500, labeled with anti-rabbit 488 Alexa) and Covance anti-Tuj1 (1:1000, labeled with anti-mouse 647).| Application Notes | IF:1:10~50 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
