Application
(LEFT) Western blot analysis of GABARAP (arrow) using rabbit polyclonal Autophagy GABARAP Antibody (Y95) (RB11847). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GABARAP gene (Lane 2) (Origene Technologies).Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Autophagy GABARAP antibody (Y95), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | GABARAP Antibody (Y95) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | FLC3B |
|---|---|
| Alternative Names | GABARAP FLC3B Gamma-aminobutyric acid receptor-associated protein GABA(A) receptor-associated protein MM46 |
| Molecular Weight(MW) | 13918 Da |
| Function | May play a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Involved in apoptosis. Involved in autophagy (By similarity) |
| Tissue Specificity | Heart, brain, placenta, liver, skeletal muscle, kidney and pancreas |
| Cellular Localization | Endomembrane system (By similarity). Cytoplasm, cytoskeleton (By similarity). Golgi apparatus membrane (By similarity). Cytoplasmic vesicle, autophagosome. Note=Largely associated with intracellular membrane structures including the Golgi apparatus and postsynaptic cisternae. Colocalizes with microtubules (By similarity) |
| Entrez Gene | 11337 |
|---|
Application
(LEFT) Western blot analysis of GABARAP (arrow) using rabbit polyclonal Autophagy GABARAP Antibody (Y95) (RB11847). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GABARAP gene (Lane 2) (Origene Technologies).
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Autophagy GABARAP antibody (Y95), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein A affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
