Application
Western blot analysis of anti-GADD45A Antibody (N-term) in Y79 cell line lysates (35ug/lane). GADD45A (arrow) was detected using the purified Pab.Application
Western blot analysis of GADD45A (arrow) using rabbit polyclonal GADD45A Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GADD45A gene.Application
GADD45A Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | GADD45A Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | DDIT1, GADD45 |
|---|---|
| Alternative Names | GADD45A DDIT1 GADD45 Growth arrest and DNA damage-inducible protein GADD45 alpha DNA damage-inducible transcript 1 protein |
| Molecular Weight(MW) | 18336 Da |
| Function | In T-cells, functions as a regulator of p38 MAPKs by inhibiting p88 phosphorylation and activity (By similarity). Might affect PCNA interaction with some CDK (cell division protein kinase) complexes; stimulates DNA excision repair in vitro and inhibits entry of cells into S phase |
| Tissue Specificity | This GADD45A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 3~32 amino acids from the N-terminal region of human GADD45A. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 1647 |
|---|
Application
Western blot analysis of anti-GADD45A Antibody (N-term) in Y79 cell line lysates (35ug/lane). GADD45A (arrow) was detected using the purified Pab.
Application
Western blot analysis of GADD45A (arrow) using rabbit polyclonal GADD45A Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GADD45A gene.
Application
GADD45A Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
