Application
Western blot analysis of GAK (arrow) using GAK Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GAK gene (Lane 2) (Origene Technologies).Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.Application
GAK Antibody (V1155) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the GAK antibody detected the GAK protein (arrow).Application
GAK Antibody (V1155) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the GAK antibody detected the GAK protein (arrow).| Product Name | GAK Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | GAK Cyclin-G-associated kinase |
|---|---|
| Molecular Weight(MW) | 143191 Da |
| Function | Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin- coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1 |
| Tissue Specificity | Ubiquitous. Highest in testis. |
| Cellular Localization | Cytoplasm, perinuclear region. Golgi apparatus, trans-Golgi network. Cell junction, focal adhesion (Probable). Note=Localizes to the perinuclear area and to the trans-Golgi network. Also seen on the plasma membrane, probably at focal adhesions |
| Entrez Gene | 2580 |
|---|
Application
Western blot analysis of GAK (arrow) using GAK Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GAK gene (Lane 2) (Origene Technologies).
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Application
GAK Antibody (V1155) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the GAK antibody detected the GAK protein (arrow).
Application
GAK Antibody (V1155) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the GAK antibody detected the GAK protein (arrow).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
