Application
Western blot analysis of GALE Antibody (Center) in A375 cell line lysates (35ug/lane). GALE (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human skin tissue reacted with GALE Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
GALE Antibody (Center) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of GALE Antibody (Center)(Cat#AP6902c) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | GALE Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFCIF |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | GALE UDP-glucose 4-epimerase Galactowaldenase UDP-galactose 4-epimerase |
|---|---|
| Molecular Weight(MW) | 38282 Da |
| Function | Catalyzes two distinct but analogous reactions: the epimerization of UDP-glucose to UDP-galactose and the epimerization of UDP-N-acetylglucosamine to UDP-N- acetylgalactosamine |
| Tissue Specificity | This GALE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 150~179 amino acids from the Center region of human GALE. |
| Entrez Gene | 2582 |
|---|
Application
Western blot analysis of GALE Antibody (Center) in A375 cell line lysates (35ug/lane). GALE (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human skin tissue reacted with GALE Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
GALE Antibody (Center) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of GALE Antibody (Center)(Cat#AP6902c) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
