Application
Western blot analysis of anti-GATA4 Antibody (C-term) in CEM cell line lysates (35ug/lane). GATA4(arrow) was detected using the purified Pab (1:60 dilution).Application
Western blot analysis of GATA4 (arrow) using rabbit polyclonal GATA4 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GATA4 gene (Lane 2) (Origene Technologies).Application
GATA4 Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | GATA4 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | GATA4 Transcription factor GATA-4 GATA-binding factor 4 |
|---|---|
| Molecular Weight(MW) | 44565 Da |
| Function | Transcriptional activator. Binds to the consensus sequence 5'-AGATAG-3'. Acts as a transcriptional activator of ANF in cooperation with NKX2-5 (By similarity). Promotes cardiac myocyte enlargement |
| Tissue Specificity | This GATA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 306~335 amino acids from the C-terminal region of human GATA4. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 2626 |
|---|
Application
Western blot analysis of anti-GATA4 Antibody (C-term) in CEM cell line lysates (35ug/lane). GATA4(arrow) was detected using the purified Pab (1:60 dilution).
Application
Western blot analysis of GATA4 (arrow) using rabbit polyclonal GATA4 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GATA4 gene (Lane 2) (Origene Technologies).
Application
GATA4 Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
