Application
Western blot analysis of GC Antibody (Center) in mouse lung tissue lysates (35ug/lane). GC (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human Skin reacted with GC Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
GC Antibody (Center) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | GC Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | GC, GLUC |
|---|---|
| Alternative Names | GBA GC GLUC Glucosylceramidase Acid beta-glucosidase Alglucerase Beta-glucocerebrosidase D-glucosyl-N-acylsphingosine glucohydrolase Imiglucerase |
| Molecular Weight(MW) | 59716 Da |
| Tissue Specificity | This GC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 344~373 amino acids from the Center region of human GC. |
| Cellular Localization | Lysosome membrane; Peripheral membrane protein; Lumenal side. Note=Interaction with saposin-C promotes membrane association. Targeting to lysosomes occurs through an alternative MPR-independent mechanism via SCARB2 |
| Entrez Gene | 2629 |
|---|
Application
Western blot analysis of GC Antibody (Center) in mouse lung tissue lysates (35ug/lane). GC (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human Skin reacted with GC Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
GC Antibody (Center) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
