Application
Western blot analysis of GCAT antibody (Center) in mouse liver tissue lysates (35ug/lane). GCAT (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human brain reacted with GCAT Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of HepG2 cells using GCAT Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of GCAT (arrow) using rabbit polyclonal GCAT Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GCAT gene.| Product Name | GCAT Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KBL |
|---|---|
| Alternative Names | GCAT KBL 2-amino-3-ketobutyrate coenzyme A ligase mitochondrial Aminoacetone synthase Glycine acetyltransferase |
| Molecular Weight(MW) | 45285 Da |
| Tissue Specificity | Strongly expressed in heart, brain, liver and pancreas. Also found in lung |
| Cellular Localization | Mitochondrion (By similarity). Nucleus. Note=Translocates to the nucleus upon cold and osmotic stress |
| Entrez Gene | 23464 |
|---|
Application
Western blot analysis of GCAT antibody (Center) in mouse liver tissue lysates (35ug/lane). GCAT (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human brain reacted with GCAT Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of HepG2 cells using GCAT Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of GCAT (arrow) using rabbit polyclonal GCAT Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GCAT gene.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
