Application
Western blot analysis of GCDH Antibody (N-term) in mouse liver tissue lysates (35ug/lane). GCDH (arrow) was detected using the purified Pab.Application
GCDH Antibody (N-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | GCDH Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | GCDH Glutaryl-CoA dehydrogenase mitochondrial |
|---|---|
| Molecular Weight(MW) | 48127 Da |
| Function | Catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA and CO(2) in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism. It uses electron transfer flavoprotein as its electron acceptor. Isoform Short is inactive |
| Tissue Specificity | Isoform 1 and isoform 2 are expressed in fibroblasts and liver |
| Cellular Localization | Mitochondrion matrix. |
| Entrez Gene | 2639 |
|---|
Application
Western blot analysis of GCDH Antibody (N-term) in mouse liver tissue lysates (35ug/lane). GCDH (arrow) was detected using the purified Pab.
Application
GCDH Antibody (N-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
