Application
Western blot analysis of hGCK-S418 in Jurkat cell line lysates (35ug/lane). GCK (arrow) was detected using the purified Pab.Application
Western blot analysis of GCK (arrow) using rabbit polyclonal hGCK-S418 . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GCK gene.| Product Name | GCK Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | GCK Glucokinase Hexokinase type IV Hexokinase-4 Hexokinase-D |
|---|---|
| Molecular Weight(MW) | 52191 Da |
| Function | Catalyzes the initial step in utilization of glucose by the beta-cell and liver at physiological glucose concentration Glucokinase has a high Km for glucose, and so it is effective only when glucose is abundant. The role of GCK is to provide G6P for the synthesis of glycogen. Pancreatic glucokinase plays an important role in modulating insulin secretion. Hepatic glucokinase helps to facilitate the uptake and conversion of glucose by acting as an insulin-sensitive determinant of hepatic glucose usage |
| Tissue Specificity | Isoform 1 is expressed in pancreas. Isoform 2 and isoform 3 is expressed in liver |
| Entrez Gene | 2645 |
|---|
Application
Western blot analysis of hGCK-S418 in Jurkat cell line lysates (35ug/lane). GCK (arrow) was detected using the purified Pab.
Application
Western blot analysis of GCK (arrow) using rabbit polyclonal hGCK-S418 . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GCK gene.| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
