Application
GCLC Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the GCLC antibody detected the GCLC protein (arrow).Application
GCLC Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human esophageal carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GCLC Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of GCLC Antibody (N-term)(Cat#AP11943a) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
GCLC Antibody (N-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.| Product Name | GCLC Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIFFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | GLCL, GLCLC |
|---|---|
| Alternative Names | GCLC GLCL GLCLC Glutamate--cysteine ligase catalytic subunit GCS heavy chain Gamma-ECS Gamma-glutamylcysteine synthetase |
| Molecular Weight(MW) | 72766 Da |
| Tissue Specificity | This GCLC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 6-34 amino acids from the N-terminal region of human GCLC. |
| Entrez Gene | 2729 |
|---|---|
| Nucleotide Accession | NP_001489.1 |
Application
GCLC Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the GCLC antibody detected the GCLC protein (arrow).
Application
GCLC Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human esophageal carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GCLC Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of GCLC Antibody (N-term)(Cat#AP11943a) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
GCLC Antibody (N-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
