Application
Western blot analysis of GEFT Antibody (Center) in mouse liver tissue lysates (35ug/lane). GEFT (arrow) was detected using the purified Pab.Application
GEFT Antibody (Center) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GEFT Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | GEFT Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | GEFT |
|---|---|
| Alternative Names | ARHGEF25 GEFT Rho guanine nucleotide exchange factor 25 Guanine nucleotide exchange factor GEFT Rac/Cdc42/Rho exchange factor GEFT RhoA/Rac/Cdc42 guanine nucleotide exchange factor GEFT p63RhoGEF |
| Molecular Weight(MW) | 63843 Da |
| Function | May play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. It works as a guanine nucleotide exchange factor for Rho family of small GTPases. Links specifically G alpha q/11- coupled receptors to RHOA activation. May be an important regulator of processes involved in axon and dendrite formation. In neurons seems to be an exchange factor primarily for RAC1 Involved in skeletal myogenesis (By similarity) |
| Tissue Specificity | Isoform 1 and isoform 2 are highly expressed in excitable tissues, such as brain, heart and muscle. Also detected in kidney and liver |
| Cellular Localization | Cell membrane (By similarity). Cytoplasm, myofibril, sarcomere. Note=Highly colocalizes with actin regions |
| Entrez Gene | 115557 |
|---|
Application
Western blot analysis of GEFT Antibody (Center) in mouse liver tissue lysates (35ug/lane). GEFT (arrow) was detected using the purified Pab.
Application
GEFT Antibody (Center) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GEFT Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
