Application
GEM Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the GEM antibody detected the GEM protein (arrow).Application
GEM Antibody (C-term) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the GEM antibody detected the GEM protein (arrow).Application
GEM Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GEM Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
GEM Antibody (C-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of GEM Antibody (C-term)(Cat#AP11235b) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).| Product Name | GEM Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFCIF |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIR |
|---|---|
| Alternative Names | GEM KIR GTP-binding protein GEM GTP-binding mitogen-induced T-cell protein RAS-like protein KIR |
| Molecular Weight(MW) | 33949 Da |
| Function | Could be a regulatory protein, possibly participating in receptor-mediated signal transduction at the plasma membrane. Has guanine nucleotide-binding activity but undetectable intrinsic GTPase activity |
| Tissue Specificity | Most abundant in thymus, spleen, kidney, lung, and testis. Less abundant in heart, brain, liver and skeletal muscle |
| Cellular Localization | Cell membrane; Peripheral membrane protein; Cytoplasmic side |
Application
GEM Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the GEM antibody detected the GEM protein (arrow).
Application
GEM Antibody (C-term) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the GEM antibody detected the GEM protein (arrow).
Application
GEM Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GEM Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
GEM Antibody (C-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of GEM Antibody (C-term)(Cat#AP11235b) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
